DSMZ 408 . PSYCHRILYOBACTER SP. MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(BD Bacto)
10.0 g 10 -
Yeast extract
(OXOID)
3.0 g 3 -
Casamino acids
(BD Bacto)
10.0 g 10 -
L-Glutamic acid 20.0 g 20 135.934
NH4Cl 2.0 g 2 37.39
K2HPO4 x 3 H2O 2.0 g 2 11.483
NaCl 25.0 g 25 427.789
Modified Wolin's mineral solution 10.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve cysteine, adjust pH to 7.0 and dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
L-Glutamic acid 20 135.934
Peptone 10 -
Casamino acids 10 -
Yeast extract 3 -
NH4Cl 2 37.39
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Cultivation metadata from 3 strains BacDive

phyla 1 2 strains
oxygen anaerobe 3 strains
temperature psychrophilic 3 strains