Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(fresh, washed, peeled and sliced)
200.0 g 200 -
CaCO3 2.0 g 2 19.982
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
D-Glucose 6.0 g 6 33.304
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
Distilled water 1000.0 ml - -
1 Mix potatoes and CaCO3 then autoclave 30 min at 121°C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Adjust pH of complete medium to 6.8.
2 Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium:
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
100.0 ml - -
Sodium resazurin
(0.1% w/v)
0.1 ml 1.0e-4 -
1 Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121°C.


Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 17

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Potato 200 -
D-Glucose 6 33.304
CaCO3 2 19.982
L-Cysteine HCl x H2O 0.5 2.847
Sodium resazurin 5.0e-4 -
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Cultivation metadata from 17 strains BacDive

phyla 1 17 strains
oxygen anaerobe 17 strains
temperature mesophilic 17 strains