411 .
CLOSTRIDIUM ACETOBUTYLICUM MEDIUM
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Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Potato
(fresh, washed, peeled and sliced) |
200.0 | g | ||
|
|
CaCO3 | 2.0 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
D-Glucose | 6.0 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix potatoes and CaCO3 then autoclave 30 min at 121°C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Adjust pH of complete medium to 6.8. | |||||
| 2 Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium: | |||||
Milk Medium #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Milk
(fresh) |
100.0 | ml | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.1 | ml | ||
| 1 Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121°C. | |||||