Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone | 2.000 | g | 2 | - | |
Yeast extract | 2.000 | g | 2 | - | |
Glucose | 4.000 | g | 4 | 22.203 | |
K2HPO4 | 0.225 | g | 0.225 | 1.292 | |
KH2PO4 | 0.255 | g | 0.255 | 1.874 | |
(NH4)2SO4 | 0.255 | g | 0.255 | 1.93 | |
NaCl | 0.500 | g | 0.5 | 8.556 | |
MgSO4 x 7 H2O | 0.100 | g | 0.1 | 0.406 | |
CaCl2 x 2 H2O | 0.070 | g | 0.07 | 0.476 | |
Na-acetate | 5.000 | g | 5 | 60.951 | |
Wolin's vitamin solution | 10.000 | ml | - | - | |
Modified Wolin's mineral solution | 10.000 | ml | - | - | |
Resazurin | 1.000 | mg | 1.0e-3 | 0.004 | |
NaHCO3 | 6.000 | g | 6 | 71.423 | |
L-Cysteine HCl x H2O | 0.500 | g | 0.5 | 2.847 | |
Distilled water | 1000.000 | ml | - | - | |
1 Autoclave under 2 bar N2:CO2 (80:20). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Last modified: | 15.06.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 6 | 71.423 |
Na-acetate | 5 | 60.951 |
Glucose | 4 | 22.203 |
Yeast extract | 2 | - |
Trypticase peptone | 2 | - |
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