DSMZ 412 . ACETOMICROBIUM FAECALIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 2.000 g 2 -
Yeast extract 2.000 g 2 -
Glucose 4.000 g 4 22.203
K2HPO4 0.225 g 0.225 1.292
KH2PO4 0.255 g 0.255 1.874
(NH4)2SO4 0.255 g 0.255 1.93
NaCl 0.500 g 0.5 8.556
MgSO4 x 7 H2O 0.100 g 0.1 0.406
CaCl2 x 2 H2O 0.070 g 0.07 0.476
Na-acetate 5.000 g 5 60.951
Wolin's vitamin solution 10.000 ml - -
Modified Wolin's mineral solution 10.000 ml - -
Resazurin 1.000 mg 1.0e-3 0.004
NaHCO3 6.000 g 6 71.423
L-Cysteine HCl x H2O 0.500 g 0.5 2.847
Distilled water 1000.000 ml - -
1 Autoclave under 2 bar N2:CO2 (80:20).
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 170 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 15.06.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 6 71.423
Na-acetate 5 60.951
Glucose 4 22.203
Yeast extract 2 -
Trypticase peptone 2 -
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Cultivation metadata from 7 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
pH 4 - 6 1 strain
phyla 1 7 strains
oxygen anaerobe 6 strains
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