414
414 .
ACIDAMINOCOCCUS FERMENTANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Adlercreutzia caecicola DSM 22242
* Modification: Plus rumen fluid (1%); obligately anaerobic
Growth conditions: 37 °C
Main sol. 414 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 5 | - | |
| Casamino acids | 10.0 | g | 10 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| Na glutamate | 4.0 | g | 4 | 23.653 | |
| Arginine | 1.0 | g | 1 | 5.741 | |
| Glycine | 1.0 | g | 1 | 13.321 | |
| DL-Tryptophan | 0.1 | g | 0.1 | 0.49 | |
| KH2PO4 | 2.0 | g | 2 | 14.697 | |
| Tween 80 | 0.5 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Clarified rumen fluid* | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 7.0 - 7.2. | |||||
| 2 Prepare and distribute medium anaerobically using the Hungate technique | |||||
Solution occurs in 28
other media (first defined in Medium 1310).
| 1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C. |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Casamino acids | 10 | - |
| Glucose | 5 | 27.753 |
| Yeast extract | 5 | - |
| Trypticase peptone | 5 | - |
| Na glutamate | 4 | 23.653 |
| Show more | ||
