DSMZ 429b . CHOCOLATE AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Selected strain: Granulicatella balaenopterae DSM 15827

This strain uses the standard medium without modifications.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Horse blood OR Sheep blood 10 % 100 -
1 Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80°C for 10 min. Mix well and pour plates after cooling to 50°C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1.
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g - -
Meat extract 3 g - -
Agar , for solid medium 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
Solution occurs in 5 other media (first defined in Medium 215).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 12
Strains with modifications: 3
Granulicatella balaenopterae DSM 15827

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 100 -
Sheep blood 100 -
Columbia agar base - -

Cultivation metadata from 12 strains BacDive

phyla 4 12 strains
oxygen microaerophile 9 strains
oxygen anaerobe 4 strains
oxygen aerobe 2 strains
oxygen facultative anaerobe 1 strain
Show more