DSMZ 429b . CHOCOLATE AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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* Modified for Vespertiliibacter pulmonis DSM 27238

Plus Vitox (OXOID); aerobic plus 5% CO2

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Horse blood OR Sheep blood 10 % 100 -
1 Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80°C for 10 min. Mix well and pour plates after cooling to 50°C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1.
Solution occurs in 20 other media (first defined in Medium 1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Agar , for solid medium 15 g 15 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.
2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
Solution occurs in 5 other media (first defined in Medium 215).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Gas composition

  • 5 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 12
Strains with modifications: 3
Vespertiliibacter pulmonis DSM 27238

  5% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 100 -
Sheep blood 100 -
Columbia agar base - -

Cultivation metadata from 12 strains BacDive

phyla 4 12 strains
oxygen microaerophile 9 strains
oxygen anaerobe 4 strains
oxygen aerobe 2 strains
oxygen facultative anaerobe 1 strain
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