This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Columbia agar base
(dissolved in 70 % artificial seawater) |
1000.0 | ml | 700 | - | |
Agar | 2.0 | g/l | 2 | 5.947 | |
Horse blood OR Sheep blood | 4.0 | % | 40 | - | |
Glucose | 1.0 | % | 10 | 55.506 | |
FeCl3 | 2.0 | mM | 0.324 | 2 | |
Cysteine | 0.1 | % | 1 | 8.254 | |
1 Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Casein peptone | 15 | g | - | - | |
Soy peptone | 5 | g | - | - | |
NaCl | 5 | g | - | - | |
Agar | 15 | g | - | - | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
Distilled water | 1000 | ml | - | - |
Last modified: | 19.11.21 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Columbia agar base | 700 | - |
Horse blood | 40 | - |
Sheep blood | 40 | - |
Glucose | 10 | 55.506 |
Agar | 2 | 5.947 |
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