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429d. FRANCISELLA HALIOTICIDA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 , strain 1000.0 ml - -
2 70.0 % 700 -
3 2.0 g 2 -
6 1.0 % 10 55.508
7 2.0 mM 0.324 2
8 0.1 % 1 8.254
Instructions:
  1. Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or medium 215.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1
Francisella halioticida DSM 23729

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Artificial Sea water 700 -
Glucose 10 55.508
Agar 2 -
Cysteine 1 8.254
FeCl3 0.324 2
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain

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