429d .
FRANCISELLA HALIOTICIDA MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia agar base
(dissolved in 70 % artificial seawater) |
1000.0 | ml | ||
|
|
Agar | 2.0 | g/l | ||
|
|
Horse blood | 4.0 | % | ||
|
|
Glucose | 1.0 | % | ||
|
|
FeCl3 | 2.0 | mM | ||
|
|
Cysteine | 0.1 | % | ||
| 1 Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. | |||||
Main sol. 220 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 15 | g | ||
|
|
Soy peptone | 5 | g | ||
|
|
NaCl | 5 | g | ||
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Agar | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||