Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Beef extract | 5.0 | g | 5 | - | |
Trypticase
(BBL) |
5.0 | g | 5 | - | |
Yeast extract | 10.0 | g | 10 | - | |
Mineral solution | 40.0 | ml | - | - | |
(NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
Glucose | 5.0 | g | 5 | 27.753 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Haemin
(dissolved in 0.5 ml 1 N NaOH) |
5.0 | mg | 0.005 | 0.008 | |
Vitamin K1
(0.5% sol. in ethanol) |
0.2 | ml | 1.0e-3 | 0.002 | |
L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
Distilled water | 960.0 | ml | - | - | |
Bovine serum
(made anaerobic by preincubation in a GasPak container over-night) |
5.0 | % | 50 | - | |
1 Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. | |||||
2 Adjust pH to 6.9. | |||||
3 Before use add 5% (v/v) bovine serum (made anaerobic by preincubation in a GasPak container over-night). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.30 | g | 0.3 | 2.041 |
MgSO4 x 7 H2O | 0.48 | g | 0.48 | 1.947 |
K2HPO4 | 1.00 | g | 1 | 5.741 |
KH2PO4 | 1.00 | g | 1 | 7.348 |
NaHCO3 | 10.00 | g | 10 | 119.038 |
NaCl | 2.00 | g | 2 | 34.223 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 17.02.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Description:
Published for cultivation of Prevotella dentalis, |
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