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441. DIAZOTROPHIC MEDIUM (RBA)

Culture medium recipe

Vol.
×
  • RBA is an ammonium-free medium which has successfully been used for the isolation, growth and purity check of a broad spectrum of nitrogen fixing bacteria (Ref. 3363). For microaerophilic nitrogen-fixing bacteria use semisolid medium with 0.3% end concentration of agar and incubate the liquid cultures under 10%(v/v) air and 90%(v/v) N2.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 950.0 ml - -
2 Solution B 50.0 ml - -
3 Standard vitamin solution
(filter-sterilized)
5.0 ml - -
4 , might need strain adjustment 0.3 % - -
Instructions:
  1. Sterilize solution A separately at 121°C for 15 min., cool to 50°C and then mix aseptically with filter-sterilized solution B and 5.0 ml of filter-sterilized standard vitamin solution (see medium 428).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.100 g 0.105 0.771
2 0.900 g 0.944 5.422
3 0.100 g 0.105 1.795
4 0.100 g 0.105 0.714
5 0.100 g 0.105 0.426
6 0.005 g 0.005 0.022
7 0.005 g 0.005 0.037
8 0.005 g 0.005 0.031
9 0.010 g 0.01 0.038
10 0.050 g 0.052 -
11 Trace element solution SL-6 3.000 ml - -
12 950.000 ml 995.065 55234.4
13
optional
15.000 g 15.74 -
Instructions:
  1. Adjust pH to 7.3.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 20 123.417
2 2 g 40 -
3 1 g 20 181.745
4 2 g 40 219.573
5 2 g 40 222.03
6 50 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 7.3.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8
Solution occurs in 6 other media (first defined in medium 1664 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.0 mg 0.1 0.266
2 50.0 mg 0.5 1.339
3 50.0 mg 0.5 4.061
4 50.0 mg 0.5 2.431
5 50.0 mg 0.5 1.049
6 0.1 mg 1.0e-3 0.004
7 0.2 mg 0.002 0.005
8 1.0 mg 0.01 0.006
9 100.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Filter

Associated strains

All strains for this medium: 49
Strains with modifications: 1
Azorhizophilus paspali DSM 376

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
14.878 -
D-Glucose 1.99 11.046
D-Mannitol 1.99 10.924
Malate 1.99 -
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Cultivation metadata from 49 strains BacDive

phyla 2 49 strains
temperature mesophilic 49 strains

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508. CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) 66 %
644. CATENOCOCCUS MEDIUM 65 %

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