446
446 .
MINERAL MEDIUM WITH 2,4-DICHLORO- PHENOXYACETIC ACID
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 446 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 442 | 1000.00 | ml | - | - | |
| 2,4-Dichlorophenoxyacetic acid | 0.05 | % | 0.5 | - | |
| 1 To medium 442 add 0.05% 2,4-D as carbon source instead of toluene, sterilized by filtration. | |||||
Solution occurs in 6
other media (first defined in Medium 442).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 12 H2O | 9.0 | g | 9 | 63.399 | |
| KH2PO4 | 1.5 | g | 1.5 | 11.023 | |
| NH4Cl | 2.0 | g | 2 | 37.39 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| Ferric ammonium citrate | 5.0 | mg | 0.005 | - | |
| MnSO4 x H2O | 3.0 | mg | 0.003 | 0.018 | |
| ZnSO4 x 7 H2O | 0.2 | mg | 2.0e-4 | 7.0e-4 | |
| CoSO4 | 10.0 | µg | 1.0e-5 | 6.5e-5 | |
|
Nitrilotriacetic acid
(NTA) |
10.0 | mg | 0.01 | 0.052 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 One drop of toluene is evaporated in a desiccator so that the atmosphere is saturated. 0.1% succinate may be added to the medium as additional carbon source. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |