Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.209 | 1.537 | |
NaCl | 1.00 | g | 1.046 | 17.899 | |
KCl | 0.50 | g | 0.523 | 7.016 | |
MgSO4 x 7 H2O | 0.50 | g | 0.523 | 2.122 | |
CaCl2 x 2 H2O | 0.15 | g | 0.157 | 1.067 | |
NH4Cl | 0.25 | g | 0.262 | 4.889 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Resazurin | 1.00 | mg | 0.001 | 0.005 | |
Na2SeO3 x 5 H2O
(0.26 g/ 1000 ml 10 mM NaOH) |
1.00 | ml | 0.003 | 0.01 | |
Glycine | 2.00 | g | 2.092 | 27.868 | |
Distilled water | 950.00 | ml | - | - | |
1 pH 7.2 to 7.4 | |||||
2 Before inoculation add per 100 ml of medium: | |||||
Biotin
(10 mg/l, autoclave for 10 minutes under N2) |
0.20 | ml | - | - | |
NaHCO3
(84 g/l, autoclaved under CO2) |
3.00 | ml | - | - | |
Na2S
(60 g/l, autoclaved under N2) |
1.00 | ml | - | - | |
3 Prepare and distribute medium anaerobically by using the Hungate technique. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.2 - 7.4 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Glycine | 2.092 | 27.868 |
NaCl | 1.046 | 17.899 |
KCl | 0.523 | 7.016 |
MgSO4 x 7 H2O | 0.523 | 2.122 |
NH4Cl | 0.262 | 4.889 |
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