454
454 .
EBA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Peptoclostridium acidaminophilum DSM 3953
This strain uses the standard medium without modifications.
Growth conditions: 34 °C
Main sol. 454 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.209 | 1.537 | |
| NaCl | 1.00 | g | 1.046 | 17.899 | |
| KCl | 0.50 | g | 0.523 | 7.016 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.523 | 2.122 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.157 | 1.067 | |
| NH4Cl | 0.25 | g | 0.262 | 4.889 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 0.001 | 0.005 | |
|
Na2SeO3 x 5 H2O
(0.26 g/ 1000 ml 10 mM NaOH) |
1.00 | ml | 0.003 | 0.01 | |
| Glycine | 2.00 | g | 2.092 | 27.868 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 pH 7.2 to 7.4 | |||||
| 2 Before inoculation add per 100 ml of medium: | |||||
|
Biotin
(10 mg/l, autoclave for 10 minutes under N2) |
0.20 | ml | - | - | |
|
NaHCO3
(84 g/l, autoclaved under CO2) |
3.00 | ml | - | - | |
|
Na2S
(60 g/l, autoclaved under N2) |
1.00 | ml | - | - | |
| 3 Prepare and distribute medium anaerobically by using the Hungate technique. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.2 - 7.4 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Peptoclostridium acidaminophilum DSM 3953
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glycine | 2.092 | 27.868 |
| NaCl | 1.046 | 17.899 |
| KCl | 0.523 | 7.016 |
| MgSO4 x 7 H2O | 0.523 | 2.122 |
| NH4Cl | 0.262 | 4.889 |
| Show more | ||
