455
455 .
AMB MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 455 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 4.00 | g | 4 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
|
Starch
(soluble) |
1.00 | g | 1 | - | |
| Meat infusion | 25.00 | g | 25 | - | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| Cysteine | 1.00 | g | 1 | 8.254 | |
| CaCl2 | 0.01 | g | 0.01 | 0.09 | |
| MgSO4 | 0.20 | g | 0.2 | 1.662 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| K2HPO4 | 15.00 | g | 15 | 86.12 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH: 6.9. | |||||
| 2 Prepare and distribute medium anaerobically by using the Hungate technique. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Meat infusion | 25 | - |
| K2HPO4 | 15 | 86.12 |
| Glucose | 5 | 27.753 |
| Yeast extract | 5 | - |
| Trypticase peptone | 4 | - |
| Show more | ||
