DSMZ 455 . AMB MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Clostridium moniliforme DSM 3984

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 4.00 g 4 -
Yeast extract 5.00 g 5 -
Starch
(soluble)
1.00 g 1 -
Meat infusion 25.00 g 25 -
Glucose 5.00 g 5 27.753
Cysteine 1.00 g 1 8.254
CaCl2 0.01 g 0.01 0.09
MgSO4 0.20 g 0.2 1.662
(NH4)2SO4 1.00 g 1 7.568
K2HPO4 15.00 g 15 86.12
Distilled water 1000.00 ml - -
1 pH: 6.9.
2 Prepare and distribute medium anaerobically by using the Hungate technique.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Hungate tubes
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Associated strains

All strains for this medium: 3
Clostridium moniliforme DSM 3984

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Meat infusion 25 -
K2HPO4 15 86.12
Glucose 5 27.753
Yeast extract 5 -
Trypticase peptone 4 -
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Cultivation metadata from 3 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature mesophilic 4 strains