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DSMZ

DSMZ 457b . MEDIUM WITH FLUORANTHENE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Pseudomonas frederiksbergensis DSM 13022

* Modification: , prepare medium 457 with 0.3 g/l casamino acids. Add phenanthrene instead of fluoranthene to the culture vessel. 0.1 ml phenanthrene stock solution in acetone (5 mg/ml) are used per flask.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C

Main sol. 457b #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 457 1000.0 ml - -
Tween 80 200.0 mg/l 0.2 0.153
Casamino acids* 0.3 g/l - -
1 For DSM 7526, prepare medium 457 with 200 mg/l Tween 80. Prepare a solution of 2 g/l fluoranthene in acetone and filter sterilize using a cellulose filter membrane. Add 1 ml of this stock solution to the sterilized culture flask. Let acetone evaporate. Add 20 ml medium to the flask. Alternatively, the direct addition of about 1-2 mg fluoranthene to the medium is possible.

Medium 457 #

1000
Solution occurs in 6 other media (first defined in Medium 457).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 g 2.44 17.188
KH2PO4 1.52 g 1.52 11.17
(NH4)2SO4 0.50 g 0.5 3.784
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL-4 10.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.9.
2 Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
3 Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 05.01.22
Source: DSMZ
Synonyms: MEDIUM WITH FLUORANTHENE OR PHENANTHRENE
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Strains with modifications: 3
Pseudomonas frederiksbergensis DSM 13022

  0.3 g/l Casamino acids

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 2.44 17.188
KH2PO4 1.52 11.17
(NH4)2SO4 0.5 3.784
Casamino acids 0.3 -
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 3 strains BacDive

phyla 2 3 strains
oxygen obligate aerobe 1 strain
temperature mesophilic 3 strains