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DSMZ 458 . MEDIUM FOR BACILLUS STEAROTHERMOPHILUS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

- PHENOL DEGRADING #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.50 g 0.5 2.871
NH4Cl 1.00 g 1 18.695
MgSO4 x 7 H2O 0.02 g 0.02 0.081
Yeast extract 0.20 g 0.2 -
Casamino acids 0.10 g 0.1 -
Distilled water 900.00 ml - -
1 pH 7.4
2 Autoclave for 15 min Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l).
Trace element solution SL-4 1.00 ml - -
Phenol
(filter-sterilized, 4.7 g/l) 		100.00 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NH4Cl 1 18.695
K2HPO4 0.5 2.871
Yeast extract 0.2 -
Casamino acids 0.1 -
MgSO4 x 7 H2O 0.02 0.081
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain

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