This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
NH4Cl | 1.00 | g | 1 | 18.695 | |
MgSO4 x 7 H2O | 0.02 | g | 0.02 | 0.081 | |
Yeast extract | 0.20 | g | 0.2 | - | |
Casamino acids | 0.10 | g | 0.1 | - | |
Distilled water | 900.00 | ml | - | - | |
1 pH 7.4 | |||||
2 Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l). | |||||
Trace element solution SL-4 | 1.00 | ml | - | - | |
Phenol
(filter-sterilized, 4.7 g/l) |
100.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.4 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NH4Cl | 1 | 18.695 |
K2HPO4 | 0.5 | 2.871 |
Yeast extract | 0.2 | - |
Casamino acids | 0.1 | - |
MgSO4 x 7 H2O | 0.02 | 0.081 |
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