458 .
MEDIUM FOR BACILLUS STEAROTHERMOPHILUS
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Equipment needed:
Autoclave
Filter
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 0.50 | g | ||
|
|
NH4Cl | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.02 | g | ||
|
|
Yeast extract | 0.20 | g | ||
|
|
Casamino acids | 0.10 | g | ||
|
|
Distilled water | 900.00 | ml | ||
| 1 pH 7.4 | |||||
| 2 Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l). | |||||
|
|
Trace element solution SL-4 | 1.00 | ml | ||
|
|
Phenol
(filter-sterilized, 4.7 g/l) |
100.00 | ml | ||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||