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DSMZ

DSMZ 461 . MINERAL MEDIUM (NAGEL AND ANDREESEN)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 461 #

ml
Solution occurs in 3 other media (first defined in Medium 461).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.00 ml - -
Na2HPO4 x 2 H2O 1.45 g 1.441 10.153
KH2PO4 0.25 g 0.249 1.826
CaCl2 0.01 g 0.01 0.09
MgSO4 x 7 H2O 0.50 g 0.497 2.017
MnSO4 0.01 g 0.01 0.066
NH4Cl 0.30 g 0.298 5.575
NaCl 0.05 g 0.05 0.85
Vitamin solution 5.00 ml - -
Trace element solution SL-10 1.00 ml - -
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 15
Strains with modifications: 14

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.441 10.153
MgSO4 x 7 H2O 0.497 2.017
NH4Cl 0.298 5.575
KH2PO4 0.249 1.826
NaCl 0.05 0.85
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Cultivation metadata from 15 strains BacDive

pH 6 - 8 1 strain
phyla 2 15 strains
oxygen aerobe 3 strains
oxygen obligate aerobe 2 strains
oxygen anaerobe 1 strain
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