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DSMZ

DSMZ 461a . MINERAL MEDIUM WITH QUINOLINE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 461a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 461 1000 ml - -
Quinoline solution 100 ml - -
1 Prepare an emulsion of 3 g/l quinoline in 50 mM phosphate buffer by stirring or ultrasonication. Autoclave in a gas tight vessel. Add 100 ml of this emulsion to 1 l autoclaved medium 461.

Medium 461 #

1000
Solution occurs in 3 other media (first defined in Medium 461).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.00 ml - -
Na2HPO4 x 2 H2O 1.45 g 1.441 10.153
KH2PO4 0.25 g 0.249 1.826
CaCl2 0.01 g 0.01 0.09
MgSO4 x 7 H2O 0.50 g 0.497 2.017
MnSO4 0.01 g 0.01 0.066
NH4Cl 0.30 g 0.298 5.575
NaCl 0.05 g 0.05 0.85
Vitamin solution 5.00 ml - -
Trace element solution SL-10 1.00 ml - -
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.

Quinoline solution #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
Quinoline 3 g/l 3 23.227
Phosphate buffer 50 mM - 50

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.31 9.23
MgSO4 x 7 H2O 0.452 1.833
Quinoline 0.273 2.112
NH4Cl 0.271 5.068
KH2PO4 0.226 1.66
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Cultivation metadata from 3 strains BacDive

phyla 2 3 strains
temperature mesophilic 3 strains