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DSMZ

DSMZ 461b . MINERAL MEDIUM WITH PYRROLIC ACID

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Arthrobacter sp. DSM 6386

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 461b #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 461 1000 ml - -
Pyrrole-2-carboxylic acid solution 10 ml - -
1 Prepare and autoclave medium 461 (vitamins not necessary). Add 10 ml of pyrrolic acid solution per liter mineral medium.

Medium 461 #

1000
Solution occurs in 3 other media (first defined in Medium 461).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.00 ml - -
Na2HPO4 x 2 H2O 1.45 g 1.441 10.153
KH2PO4 0.25 g 0.249 1.826
CaCl2 0.01 g 0.01 0.09
MgSO4 x 7 H2O 0.50 g 0.497 2.017
MnSO4 0.01 g 0.01 0.066
NH4Cl 0.30 g 0.298 5.575
NaCl 0.05 g 0.05 0.85
Vitamin solution 5.00 ml - -
Trace element solution SL-10 1.00 ml - -
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5.
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source.

Pyrrole-2-carboxylic acid solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyrrole-2-carboxylic acid 10 M 1111 10000
Potassium phosphate buffer
(50 mM; pH 7.2) 		1000 ml - 50
1 Dissolve 10 mol pyrrole-2-carboxylic acid per liter potassium phosphate buffer (50 mM; pH 7.2) and sterilize the solution by membrane filtration.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Arthrobacter sp. DSM 6386

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Pyrrole-2-carboxylic acid 11 99.01
Na2HPO4 x 2 H2O 1.427 10.053
MgSO4 x 7 H2O 0.492 1.997
NH4Cl 0.295 5.52
KH2PO4 0.246 1.808
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain