This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 461 | 800 | ml | - | - | |
Nitrophenol solution | 200 | ml | - | - | |
1 Prepare and autoclave medium 461 (vitamins not necessary). Add 200 ml nitrophenol solution to 800 ml medium 461. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Distilled water | 1000.00 | ml | - | - | |
Na2HPO4 x 2 H2O | 1.45 | g | 1.441 | 10.153 | |
KH2PO4 | 0.25 | g | 0.249 | 1.826 | |
CaCl2 | 0.01 | g | 0.01 | 0.09 | |
MgSO4 x 7 H2O | 0.50 | g | 0.497 | 2.017 | |
MnSO4 | 0.01 | g | 0.01 | 0.066 | |
NH4Cl | 0.30 | g | 0.298 | 5.575 | |
NaCl | 0.05 | g | 0.05 | 0.85 | |
Vitamin solution | 5.00 | ml | - | - | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
1 Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. | |||||
2 Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
2-Nitrophenol | 0.5 | g | 0.5 | 3.594 | |
Phosphate buffer
(50 mM, pH 7.5) |
1000.0 | ml | - | 50 | |
1 Dissolve 0.5 g o-nitrophenol per liter phosphate buffer (50 mM, pH 7.5) and sterilize by filtration. |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2HPO4 x 2 H2O | 1.153 | 8.123 |
MgSO4 x 7 H2O | 0.398 | 1.613 |
NH4Cl | 0.239 | 4.46 |
KH2PO4 | 0.199 | 1.461 |
2-Nitrophenol | 0.1 | 0.719 |
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