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DSMZ

DSMZ 465g . MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Main sol. 465g #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 g 3.465 24.411
KH2PO4 1.0 g 0.99 7.276
MgCl2 x 6 H2O 0.1 g 0.099 0.487
Trace element solution SL-4 1.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare and autoclave medium. Add 5.5 g/l glycerol from a sterile solution. Add 645 mg/l cyanuric acid from a neutralized and filter sterilized stock solution and 10 ml vitamin solution (see medium 461) to the medium.
Glycerol 5.5 g/l 5.5 59.724
Cyanuric acid 645.0 mg/l 0.645 4.997
Vitamin solution 10.0 ml - -
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycerol 5.5 59.724
Na2HPO4 x 2 H2O 3.465 24.411
KH2PO4 0.99 7.276
Cyanuric acid 0.645 4.997
MgCl2 x 6 H2O 0.099 0.487
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain