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470. PSEUDOMONAS HALOPHILA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 900 ml - -
2 Solution B 100 ml - -
Instructions:
  1. Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling.
  2. Adjust pH to 7.0 with 6 M NaOH or HCl.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 46.8 g 51.942 888.776
2 39.4 g 43.729 177.418
3 1.0 g 1.11 20.749
4 5.0 g 5.549 60.258
5 Trace element solution SL-10 1.0 ml - -
6
(see medium 131)
10.0 ml - -
7 890.0 ml 986.02 54732.4
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 10 73.484
2 100 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 988.226 54854.8
NaOH 239.983 6000
NaCl 46.748 799.898
MgSO4 x 7 H2O 39.356 159.676
Glycerol 4.994 54.232
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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