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DSMZ

DSMZ 470 . PSEUDOMONAS HALOPHILA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Halovibrio variabilis DSM 3050

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 470 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
1 Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling.
2 Adjust pH to 7.0 with 6 M NaOH or HCl.

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 46.8 g 51.942 888.814
MgSO4 x 7 H2O 39.4 g 43.729 177.418
NH4Cl 1.0 g 1.11 20.749
Glycerol 5.0 g 5.549 60.261
Trace element solution SL-10 1.0 ml - -
Vitamin solution 10.0 ml - -
Distilled water 890.0 ml - -

Solution B #

100
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1 g 10 73.484
Distilled water 100 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Halovibrio variabilis DSM 3050

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 46.753 800.021
MgSO4 x 7 H2O 39.361 159.694
Glycerol 4.995 54.241
NH4Cl 0.999 18.676
KH2PO4 0.999 7.341
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain