This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 900 | ml | - | - | |
Solution B | 100 | ml | - | - | |
1 Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling. | |||||
2 Adjust pH to 7.0 with 6 M NaOH or HCl. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaCl | 46.8 | g | 51.942 | 888.814 |
MgSO4 x 7 H2O | 39.4 | g | 43.729 | 177.418 |
NH4Cl | 1.0 | g | 1.11 | 20.749 |
Glycerol | 5.0 | g | 5.549 | 60.261 |
Trace element solution SL-10 | 1.0 | ml | - | - |
Vitamin solution | 10.0 | ml | - | - |
Distilled water | 890.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
KH2PO4 | 1 | g | 10 | 73.484 |
Distilled water | 100 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.00 | mg | 0.002 | 0.008 |
Folic acid | 2.00 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.00 | mg | 0.01 | 0.049 |
Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | 0.013 |
Riboflavin | 5.00 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.00 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 |
p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 |
Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 46.753 | 800.021 |
MgSO4 x 7 H2O | 39.361 | 159.694 |
Glycerol | 4.995 | 54.241 |
NH4Cl | 0.999 | 18.676 |
KH2PO4 | 0.999 | 7.341 |
Show more |