MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 471 . ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 471 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
Solution C 2 ml - -
1 Autoclave solutions A, B and C separately, combine, adjust pH to 8.2.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 5.12 g 10.24 58.791
KH2PO4 1.50 g 3 22.045
Distilled water 500.00 ml - -
1 Adjust pH to 9.0 with KOH.

Solution B #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.6 11.217
CaCl2 0.01 g 0.02 0.18
MgSO4 x 7 H2O 0.20 g 0.4 1.623
KNO3 2.00 g 4 39.564
Na-benzoate 0.92 g 1.84 12.769
Distilled water 500.00 ml - -

Solution C #

2
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-4 2 ml - -

Trace element solution SL-4 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -
1 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6.
2 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.2
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2HPO4 5.11 29.337
KNO3 1.996 19.742
KH2PO4 1.497 11.001
Na-benzoate 0.918 6.372
NH4Cl 0.299 5.597
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 2 strains