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DSMZ

DSMZ 471 . ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Pseudomonas stutzeri DSM 7136

* Modification: (instead of benzoate, add 3 g/l sodium acetate, pH 8.0); anaerobic

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C

Main sol. 471 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
Solution C 2 ml - -
Sodium acetate* 3 g/l - -
1 Autoclave solutions A, B and C separately, combine, adjust pH to 8.2.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 5.12 g 10.24 58.791
KH2PO4 1.50 g 3 22.045
Distilled water 500.00 ml - -
1 Adjust pH to 9.0 with KOH.

Solution B #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.6 11.217
CaCl2 0.01 g 0.02 0.18
MgSO4 x 7 H2O 0.20 g 0.4 1.623
KNO3 2.00 g 4 39.564
Na-benzoate* 0.92 g 1.84 12.769
Distilled water 500.00 ml - -

Solution C #

2
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-4 2 ml - -

Trace element solution SL-4 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
1 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6.
Distilled water 900.0 ml - -
2 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: * 8.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Pseudomonas stutzeri DSM 7136

  3.0 g/l Sodium acetate
  8.0
  Na-benzoate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
K2HPO4 5.11 29.337
Sodium acetate 3 36.572
KNO3 1.996 19.742
KH2PO4 1.497 11.001
Na-benzoate 0.918 6.372
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 2 strains