471 .
ALCALIGENES XYLOSOXYDANS MEDIUM WITH BENZOATE
What is this page?
* Medium for Pseudomonas stutzeri DSM 7136
(instead of benzoate, add 3 g/l sodium acetate, pH 8.0); anaerobic
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
* 8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
|
|
Solution C | 2 | ml | ||
|
|
Sodium acetate | 3 | g/l | ||
| 1 Autoclave solutions A, B and C separately, combine, adjust pH to 8.2. | |||||
Solution A #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 5.12 | g | ||
|
|
KH2PO4 | 1.50 | g | ||
|
|
Distilled water | 500.00 | ml | ||
| 1 Adjust pH to 9.0 with KOH. | |||||
Solution B #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NH4Cl | 0.30 | g |
|
|
CaCl2 | 0.01 | g |
|
|
MgSO4 x 7 H2O | 0.20 | g |
|
|
KNO3 | 2.00 | g |
| Na-benzoate* | 0.92 | g | |
|
|
Distilled water | 500.00 | ml |
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 0.5 | g | ||
|
|
FeSO4 x 7 H2O | 0.2 | g | ||
|
|
Trace element solution SL-6 | 100.0 | ml | ||
| 1 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6. | |||||
|
|
Distilled water | 900.0 | ml | ||
| 2 First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |