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474. MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3 g 2.727 -
2 100 ml 90.746 5037.17
3 Main sol. 457 1000 ml - -
4 16 g/l 16 -
Instructions:
  1. Prepare a suspension of 3.0 g poly-ß-hydroxybutyric acid (PHB) in 100 ml distilled water, stir over night and sonicate until a white homogenous suspension is obtained. Autoclave for 5 minutes at 121°C. Prepare double layer agar plates. First layer: medium 457 with 16.0 g agar per l. Second layer: medium 457 with agar, supplemented with 6.6 ml sterile PHB-suspension per 100 ml.
Solution occurs in 6 other media (first defined in medium 457 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.44 g 2.416 17.018
2 1.52 g 1.505 11.059
3 0.50 g 0.495 3.746
4 0.20 g 0.198 0.803
5 0.05 g 0.05 0.337
6 Trace element solution SL-4 10.00 ml - -
7 1000.00 ml 988.324 54860.2
Instructions:
  1. Adjust pH to 6.9.
  2. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling.
  3. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source.
Solution occurs in 23 other media (first defined in medium 471 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 1.723
2 0.2 g 0.2 0.719
3 Trace element solution SL-6 100.0 ml - -
4 900.0 ml 898.386 49868
Instructions:
  1. First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL-6.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: unknown
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 16 -
Poly-ß-hydroxybutyric acid 2.727 -
Na2HPO4 2.196 15.471
KH2PO4 1.368 10.054
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

Similar media

457. MINERAL MEDIUM (BRUNNER) 81 %
402. ALICYCLOBACILLUS MEDIUM 79 %
457d. MEDIUM WITH BIPHENYL 79 %
457c. MEDIUM WITH CHLOROACRYLIC ACID 77 %
473. MEDIUM WITH EDTA AS CARBON SOURCE 76 %

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