Name: ACIDIHALOBACTER PROSPERUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 477 #

Equipment needed: Autoclave Filter Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KCl	0.33	g	0.329	4.418
MgCl₂ x 6 H₂O	2.75	g	2.745	13.5
MgSO₄ x 7 H₂O	3.45	g	3.443	13.969
NH₄Cl	1.25	g	1.248	23.322
CaCl₂ x 2 H₂O	0.14	g	0.14	0.95
K₂HPO₄	0.14	g	0.14	0.802
KH₂PO₄	0.14	g	0.14	1.027
NaCl	0.50	g	0.499	8.539
Modified Wolin's mineral solution	10.00	ml	-	-
NiCl₂ x 6 H₂O (0.1% w/v)	2.00	ml	0.002	0.015
FeSO₄ x 7 H₂O (40% w/v)	50.00	ml	19.96	71.795
Distilled water	940.00	ml	-	-
1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H₂SO₄. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO₄ x 7 H₂O from a 40% (w/v) stock solution prepared with 0.2 N H₂SO₄ and filter-sterilized under 100% N₂ gas atmosphere.

Modified Wolin's mineral solution #

1000

Solution occurs in 167 other media (first defined in Medium 141).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.50	g	1.5	7.848
MgSO₄ x 7 H₂O	3.00	g	3	12.172
MnSO₄ x H₂O	0.50	g	0.5	2.958
NaCl	1.00	g	1	17.112
FeSO₄ x 7 H₂O	0.10	g	0.1	0.36
CoSO₄ x 7 H₂O	0.18	g	0.18	1.161
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.18	g	0.18	0.626
CuSO₄ x 5 H₂O	0.01	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.02	g	0.02	0.042
H₃BO₃	0.01	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.01	g	0.01	0.041
NiCl₂ x 6 H₂O	0.03	g	0.03	0.231
Na₂SeO₃ x 5 H₂O	0.30	mg	3.0e-4	0.001
Na₂WO₄ x 2 H₂O	0.40	mg	4.0e-4	0.001
Distilled water	1000.00	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Last modified:	28.11.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Defined medium
Final pH:	2.5
Equipment needed:	Autoclave, Filter, Gassing station

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All strains for this medium:	2
Strains with modifications:	1

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