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DSMZ

DSMZ 477 . ACIDIHALOBACTER PROSPERUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Acidihalobacter prosperus DSM 5130

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days 35 °C

Main sol. 477 #

ml
Equipment needed: Autoclave Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.329 4.418
MgCl2 x 6 H2O 2.75 g 2.745 13.5
MgSO4 x 7 H2O 3.45 g 3.443 13.969
NH4Cl 1.25 g 1.248 23.322
CaCl2 x 2 H2O 0.14 g 0.14 0.95
K2HPO4 0.14 g 0.14 0.802
KH2PO4 0.14 g 0.14 1.027
NaCl 0.50 g 0.499 8.539
Modified Wolin's mineral solution 10.00 ml - -
NiCl2 x 6 H2O
(0.1% w/v) 		2.00 ml 0.002 0.015
FeSO4 x 7 H2O
(40% w/v) 		50.00 ml 19.96 71.795
Distilled water 940.00 ml - -
1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 28.11.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 2.5
Equipment needed: Autoclave, Filter, Gassing station
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Acidihalobacter prosperus DSM 5130

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 19.96 71.795
MgCl2 x 6 H2O 2.745 13.5
NH4Cl 1.248 23.322
KCl 0.329 4.418
KH2PO4 0.14 1.027
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Cultivation metadata from 2 strains BacDive

halophily halotolerant 1 strain
pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 2 strains
nutrition chemolithoautotroph 1 strain
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