477 .
ACIDIHALOBACTER PROSPERUS MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Gassing station
Final pH:
2.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KCl | 0.33 | g | ||
|
|
MgCl2 x 6 H2O | 2.75 | g | ||
|
|
MgSO4 x 7 H2O | 3.45 | g | ||
|
|
NH4Cl | 1.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.14 | g | ||
|
|
K2HPO4 | 0.14 | g | ||
|
|
KH2PO4 | 0.14 | g | ||
|
|
NaCl | 0.50 | g | ||
|
|
Modified Wolin's mineral solution | 10.00 | ml | ||
|
|
NiCl2 x 6 H2O
(0.1% w/v) |
2.00 | ml | ||
|
|
FeSO4 x 7 H2O
(40% w/v) |
50.00 | ml | ||
|
|
Distilled water | 940.00 | ml | ||
| 1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.5 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Prior to inoculation add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere. | |||||
Modified Wolin's mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.18 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.18 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 x 12 H2O | 0.02 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.03 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.30 | mg | ||
|
|
Na2WO4 x 2 H2O | 0.40 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||