479
479 .
METHANOHALOPHILUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 479 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 87.00 | g | 86.139 | 1473.97 | |
| KCl | 1.50 | g | 1.485 | 19.922 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.941 | 29.22 | |
| CaCl2 x 2 H2O | 0.40 | g | 0.396 | 2.694 | |
| NH4Cl | 1.00 | g | 0.99 | 18.51 | |
| K2HPO4 x 3 H2O | 0.40 | g | 0.396 | 2.274 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.98 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.98 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.50 | g | 1.485 | 14.012 | |
| Trimethylamine-HCl | 2.00 | g | 1.98 | - | |
|
2-Mercaptoethanesulfonate
(coenzyme M) |
0.20 | g | 0.198 | 1.393 | |
| Na2S x 9 H2O | 0.25 | g | 0.248 | 1.031 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, trimethylamine, coenzyme M and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine, coenzyme M and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Solution occurs in 167
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
| Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 26.11.24 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.2 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgCl2 x 6 H2O | 5.941 | 29.22 |
| Trimethylamine-HCl | 1.98 | - |
| Trypticase peptone | 1.98 | - |
| Yeast extract | 1.98 | - |
| KCl | 1.485 | 19.922 |
| Show more | ||
