DSMZ 479a . METHANOHALOPHILUS PROFUNDI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 120.00 g 120 2053.39
KCl 1.50 g 1.5 20.121
MgCl2 x 6 H2O 6.00 g 6 29.513
CaCl2 x 2 H2O 0.40 g 0.4 2.721
NH4Cl 1.00 g 1 18.695
K2HPO4 x 3 H2O 0.40 g 0.4 2.297
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract
(OXOID)
2.00 g 2 -
Trypticase peptone
(BD BBL)
2.00 g 2 -
HEPES
(SIGMA H3375)
5.00 g 5 20.982
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Trimethylamine-HCl 2.00 g 2 -
2-Mercaptoethanesulfonic acid
(coenzyme M)
0.20 g 0.2 1.406
Na2S x 9 H2O 0.25 g 0.25 1.041
Distilled water 1000.00 ml - -
1 Dissolve ingredients except trimethylamine, coenzyme M and sulfide. Adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic; then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine, coenzyme M and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.5, if necessary.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 6 29.513
HEPES 5 20.982
Trypticase peptone 2 -
Yeast extract 2 -
Trimethylamine-HCl 2 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain