48
48 .
TRYPTONE THIOGLYCOLATE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 48 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 5.45 | g | 5.45 | 31.29 | |
| KH2PO4 | 1.20 | g | 1.2 | 8.818 | |
| MgSO4 x 7 H2O | 25.00 | mg | 0.025 | 0.101 | |
| CaCl2 x 2 H2O | 15.00 | mg | 0.015 | 0.102 | |
| FeSO4 x 7 H2O | 10.00 | mg | 0.01 | 0.036 | |
| MnCl2 x 4 H2O | 2.00 | mg | 0.002 | 0.01 | |
| CoCl2 x 6 H2O | 2.50 | mg | 0.003 | 0.019 | |
| Na2MoO4 x 2 H2O | 2.50 | mg | 0.003 | 0.01 | |
| Glucose | 20.00 | g | 20 | 111.012 | |
| Peptone | 2.00 | g | 2 | - | |
| Tryptone | 2.00 | g | 2 | 28.137 | |
| Yeast extract | 6.00 | g | 6 | - | |
| Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except glucose), adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
| 2 Add glucose to the medium after autoclaving from a sterile stock solution prepared under 100% N2 gas. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 20 | 111.012 |
| Yeast extract | 6 | - |
| K2HPO4 | 5.45 | 31.29 |
| Tryptone | 2 | 28.137 |
| Peptone | 2 | - |
| Show more | ||
