480
480 .
DESULFURELLA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Desulfurella multipotens DSM 8415
* Modification: Replace acetate with 5.00 g/l Na-butyrate.
Growth conditions: anaerobic
55 °C
Main sol. 480 #
ml
Equipment needed: Hungate tubes
Filter
Gassing station
Autoclave
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.33 | g | 0.329 | 6.157 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.24 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.329 | 1.62 | |
| KCl | 0.33 | g | 0.329 | 4.418 | |
| KH2PO4 | 0.33 | g | 0.329 | 2.42 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Yeast extract | 0.10 | g | 0.1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
|
Sulfur
(powder) |
10.00 | g | 9.98 | 311.195 | |
| Na-acetate* | 5.00 | g | 4.99 | 60.829 | |
| NaHCO3 | 2.50 | g | 2.495 | 29.7 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| DL-Dithiothreitol | 0.30 | g | 0.299 | 1.94 | |
| Na-butyrate* | 5.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, acetate, bicarbonate, vitamins, and dithiothreitol) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize by heating for 2-3 hours in a boiling water bath on each of 3 successive days. Add acetate, vitamins, and dithiothreitol from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Solutions of vitamins and dithiothreitol are sterilized by filtration. Prior to using adjust the pH of the complete medium to 6.8 - 7.0, if necessary. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 306
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 09.06.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes, Water bath |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 9.98 | 311.195 |
| Na-butyrate | 4.99 | 45.328 |
| NaHCO3 | 2.495 | 29.7 |
| NH4Cl | 0.329 | 6.157 |
| KCl | 0.329 | 4.418 |
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