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480b. TTD-MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 mod. medium 480 (medium 480b) 1000.0 ml - -
2 0.5 - -
3 2.5 - -
4
(5 % w/v)
30.0 ml/l 1.5 17.856
Instructions:
  1. Use medium 480 without yeast extract, sodium acetate and NaHCO3.
  2. Add to the medium 0. 5% casitone and 2.5% NaCl (marine source). Adjust pH to 5.7 with hydrochloric acid before sterilization. Sterilize the medium by heating for 1 h at 90 - 100°C on three successive days. Before use add 30.0 ml/l of 5%(w/v) sterile, anaerobic NaHCO3-solution, vitamins and sulfide. Final pH should be 6.5.
Equipment needed: Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.33 6.169
2 0.33 g 0.33 2.245
3 0.33 g 0.33 1.623
4 0.33 g 0.33 4.427
5 0.33 g 0.33 2.425
6 Trace element solution SL-10 1.00 ml - -
7
(0.1% w/v)
0.50 ml 5.0e-4 0.002
8
(powdered)
10.00 g 10 311.857
9 Vitamin solution 10.00 ml - -
10 0.50 g 0.5 2.082
11 1000.00 ml 998.207 55408.8
12
optional
5.00 g/l 5 45.419
Instructions:
  1. Dissolve ingredients (except sulfur, acetate, bicarbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize by heating for 2- 3 hours in a boiling water bath on each of 3 successive days. Add acetate, vitamins (sterilized by filtration)and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
  2. Look for further instructions at medium 480.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1009.18 56017.7
Sulfur 10 311.857
Na-butyrate
optional
5 45.419
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.5 2.082
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain

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