Loading ...

480d. THERMOPROTEUS UZONIENSIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 mod. medium 480 (medium 480d) 1000 ml - -
Instructions:
  1. Use medium 480, but reduce the amount of Na acetate to 1.0 g/l and add 3.0 g/l casitone. Prepare sulphide and vitamin stock solutions anaerobically. Autoclave the sulphide solution but filter sterilise the vitamins. Prepare the medium anaerobically under N2:CO2 80:20. Adjust the pH to 5.7 with HCl. Sterilise the medium by heating for 3 hours on three successive days at 90-100°C. Add the vitamins and sulphide to the sterilised medium before use. The final pH should be 5.7-6.0. Dr. Bonch-Osmoloskaya supplied the information that the strain grows better on a peptone originally available in Russia. This product is no longer available, but it appears this product gave better growth than with highly purified products from Oxoid or Difco.
Equipment needed: Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.33 6.169
2 0.33 g 0.33 2.245
3 0.33 g 0.33 1.623
4 0.33 g 0.33 4.427
5 0.33 g 0.33 2.425
6 0.10 g 0.1 -
7 Trace element solution SL-10 1.00 ml - -
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9
(powdered)
10.00 g 10 311.857
10 1.00 g 1 12.19
11 2.50 g 2.5 29.76
12 Vitamin solution 10.00 ml - -
13 0.50 g 0.5 2.082
14 1000.00 ml 998.207 55408.8
15
optional
5.00 g/l 5 45.419
16 3.00 g/l 3 -
Instructions:
  1. Dissolve ingredients (except sulfur, acetate, bicarbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize by heating for 2- 3 hours in a boiling water bath on each of 3 successive days. Add acetate, vitamins (sterilized by filtration)and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary.
  2. Look for further instructions at medium 480.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.7
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1009.18 56017.7
Sulfur 10 311.857
Na-butyrate
optional
5 45.419
Casitone 3 -
NaHCO3 2.5 29.76
Show more

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain

Similar media

480. DESULFURELLA MEDIUM 97 %
480b. TTD-MEDIUM 95 %
395a. BROCKIA LITHOTROPHICA MEDIUM 90 %
480a. DESULFURELLA MEDIUM (BRACKISH WATER) 89 %
901. ACIDOLOBUS ACETICUS MEDIUM 89 %

Latest comments

No comments for this medium.