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489 .
LACRIMISPORA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Lacrimispora aerotolerans DSM 5434
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 489 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.22 | g | 0.22 | 1.263 | |
| KH2PO4 | 0.22 | g | 0.22 | 1.617 | |
| NaCl | 0.45 | g | 0.45 | 7.7 | |
| (NH4)2SO4 | 0.45 | g | 0.45 | 3.405 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.408 | |
| MgSO4 x 7 H2O | 0.09 | g | 0.09 | 0.365 | |
| Yeast extract | 5.00 | g | 5 | - | |
| Xylan | 5.00 | g | 5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.15 | g | 0.15 | 0.854 | |
| Na2S x 9 H2O | 0.15 | g | 0.15 | 0.625 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Metadata
| Last modified: | 05.11.22 |
| Source: | DSMZ |
| Synonyms: | CLOSTRIDIUM AEROTOLERANS MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Lacrimispora aerotolerans DSM 5434
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Xylan | 5 | - |
| Yeast extract | 5 | - |
| NaCl | 0.45 | 7.7 |
| (NH4)2SO4 | 0.45 | 3.405 |
| KH2PO4 | 0.22 | 1.617 |
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