48 .
TRYPTONE THIOGLYCOLATE MEDIUM
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Selected strain: Clostridium beijerinckii DSM 51
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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K2HPO4 | 5.45 | g | ||
|
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KH2PO4 | 1.20 | g | ||
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MgSO4 x 7 H2O | 25.00 | mg | ||
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CaCl2 x 2 H2O | 15.00 | mg | ||
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FeSO4 x 7 H2O | 10.00 | mg | ||
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MnCl2 x 4 H2O | 2.00 | mg | ||
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CoCl2 x 6 H2O | 2.50 | mg | ||
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Na2MoO4 x 2 H2O | 2.50 | mg | ||
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Glucose | 20.00 | g | ||
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Peptone | 2.00 | g | ||
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Tryptone | 2.00 | g | ||
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Yeast extract | 6.00 | g | ||
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Na-thioglycolate | 0.50 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except glucose), adjust pH to 7.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
| 2 Add glucose to the medium after autoclaving from a sterile stock solution prepared under 100% N2 gas. | |||||