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DSMZ

DSMZ 490 . METHANOLOBUS OREGONENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 490 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(OXOID) 		2.0 g 1.98 -
Trypticase peptone
(BD BBL) 		2.0 g 1.98 -
NaCl 29.0 g 28.713 491.322
KCl 1.5 g 1.485 19.922
NH4Cl 1.0 g 0.99 18.51
K2HPO4 x 3 H2O 0.4 g 0.396 2.274
MgCl2 x 6 H2O 1.7 g 1.683 8.279
2-Mercaptoethanesulfonate
(coenzyme M) 		0.5 g 0.495 3.481
Modified Wolin's mineral solution 10.0 ml - -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 2.5 g 2.475 23.354
Trimethylamine-HCl 2.0 g 1.98 -
Na2S x 9 H2O 0.3 g 0.297 1.237
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, trimethylamine and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add trimethylamine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v).

Modified Wolin's mineral solution #

1000
Solution occurs in 167 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 26.11.24
Source: DSMZ
Synonyms: METHANOHALOPHILUS OREGONESE MEDIUM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 2.475 23.354
Trimethylamine-HCl 1.98 -
Yeast extract 1.98 -
Trypticase peptone 1.98 -
MgCl2 x 6 H2O 1.683 8.279
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains