DSMZ 492 . CLOSTRIDIUM METHYLPENTOSUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 954 ml - -
Solution B 20 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 20 ml - -
1 Sparge solution A with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved separately under 100% N2 gas. Solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.9 g 0.943 17.637
KH2PO4 0.9 g 0.943 6.932
NaCl 0.9 g 0.943 16.143
CaCl2 x 2 H2O 20.0 mg 0.021 0.143
MnCl2 x 4 H2O 20.0 mg 0.021 0.106
MgSO4 x 7 H2O 20.0 mg 0.021 0.085
CoCl2 x 6 H2O
(0.1% w/v)
5.0 ml 0.005 0.039
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4)
5.0 ml 0.005 0.018
ZnSO4 x 7 H2O
(0.1% w/v)
2.0 ml 0.002 0.007
CuSO4 x H2O
(0.1% w/v)
2.0 ml 0.002 0.013
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
Distilled water 940.0 ml - -
1 Adjust pH to 6.5 with KOH.
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 50 595.188
Distilled water 20 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Rhamnose 2 g 200 1218.32
Distilled water 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 1 g 50 284.68
Distilled water 20 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
L-Rhamnose 1.972 12.015
NaHCO3 0.986 11.739
L-Cysteine HCl x H2O 0.986 5.615
NH4Cl 0.888 16.593
NaCl 0.888 15.188
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain