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DSMZ

DSMZ 492 . CLOSTRIDIUM METHYLPENTOSUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 492 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 954 ml - -
Solution B 20 ml - -
Solution C 10 ml - -
Solution D 1 ml - -
Solution E 20 ml - -
1 Sparge solution A with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved separately under 100% N2 gas. Solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.0.

Solution A #

954
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.9 g 0.943 17.637
KH2PO4 0.9 g 0.943 6.932
NaCl 0.9 g 0.943 16.143
CaCl2 x 2 H2O 20.0 mg 0.021 0.143
MnCl2 x 4 H2O 20.0 mg 0.021 0.106
MgSO4 x 7 H2O 20.0 mg 0.021 0.085
CoCl2 x 6 H2O
(0.1% w/v) 		5.0 ml 0.005 0.04
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		5.0 ml 0.005 0.019
ZnSO4 x 7 H2O
(0.1% w/v) 		2.0 ml 0.002 0.007
CuSO4 x H2O
(0.1% w/v) 		2.0 ml 0.002 0.013
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.2e-4 0.002
Distilled water 940.0 ml - -
1 Adjust pH to 6.5 with KOH.

Solution B #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 1 g 50 595.188
Distilled water 20 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Rhamnose 2 g 200 1218.32
Distilled water 10 ml - -

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Solution E #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 1 g 50 284.68
Distilled water 20 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
L-Rhamnose 1.99 12.123
NaHCO3 0.995 11.845
L-Cysteine HCl x H2O 0.995 5.665
NH4Cl 0.896 16.742
NaCl 0.896 15.324
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain