DSMZ 493 . THIOMONAS (HÖ5) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.33 4.427
MgCl2 x 6 H2O 2.75 g 2.75 13.527
MgSO4 x 7 H2O 3.45 g 3.45 13.997
NH4Cl 1.25 g 1.25 23.369
CaCl2 x 2 H2O 0.14 g 0.14 0.952
K2HPO4 0.14 g 0.14 0.804
KH2PO4 0.14 g 0.14 1.029
NaCl 0.50 g 0.5 8.556
NiCl2 x 6 H2O
(0.1% w/v)
2.00 ml 0.002 0.015
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract 0.50 g 0.5 -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except yeast extract and adjust pH to 3.5 with sulfuric acid. After autoclaving add yeast extract from a sterile stock solution.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 3.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.75 13.527
NH4Cl 1.25 23.369
Yeast extract 0.5 -
KCl 0.33 4.427
KH2PO4 0.14 1.029
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain