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496. DESULFOVIBRIO HALOPHILUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 930 ml - -
2 Solution B 1 ml - -
3 Solution C 20 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
6 Solution F 1 ml - -
7 Solution G 10 ml - -
8 , may stimulate growth of some strains 20 mg 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then dispensed under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is filter-sterilized and gassed with 100% N2 gas. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.2.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10%(v/v) inoculum.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.226 22.71
2 0.20 g 0.215 1.58
3 0.30 g 0.323 6.031
4 70.00 g 75.269 1287.91
5 3.00 g 3.226 15.867
6 0.50 g 0.538 7.212
7 0.15 g 0.161 1.097
8
(0.1% w/v)
0.50 ml 5.4e-4 0.002
9 930.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 50 471.752
2 20 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.5 g 250 2230.95
2 10.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 7
Strains with modifications: 7

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
NaCl 71.283 1219.71
Na2SO4 3.055 21.508
MgCl2 x 6 H2O 3.055 15.027
Na-L-lactate 2.546 22.718
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Cultivation metadata from 7 strains BacDive

halophily slightly 1 strain
halophily not 1 strain
halophily moderate 1 strain
pH 6 - 8 3 strains
pH 4 - 6 3 strains
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