DSMZ 496 . DESULFOVIBRIO HALOPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 930 ml - -
Solution B 1 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 1 ml - -
Solution G 10 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then dispensed under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is filter-sterilized and gassed with 100% N2 gas. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 6.8 - 7.2.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% (v/v) inoculum.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.00 g 3.226 22.711
KH2PO4 0.20 g 0.215 1.58
NH4Cl 0.30 g 0.323 6.031
NaCl 70.00 g 75.269 1287.97
MgCl2 x 6 H2O 3.00 g 3.226 15.867
KCl 0.50 g 0.538 7.212
CaCl2 x 2 H2O 0.15 g 0.161 1.097
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Distilled water 930.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-L-lactate 2.5 g 250 -
Distilled water 10.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.4 g 40 166.54
Distilled water 10.0 ml - -
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 356 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 71.283 1219.77
Na2SO4 3.055 21.508
MgCl2 x 6 H2O 3.055 15.027
Na-L-lactate 2.546 -
Na2CO3 1.018 9.608
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Cultivation metadata from 7 strains BacDive

halophily moderately halophilic 2 strains
pH 6 - 8 3 strains
pH 4 - 6 2 strains
pH 8 - 10 2 strains
phyla 1 7 strains
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