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DSMZ

DSMZ 498 . PSEUDOTHERMOTOGA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Pseudothermotoga thermarum DSM 5069

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 70 °C

Main sol. 498 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD Bacto) 		0.50 g 0.488 -
Modified Wolin's mineral solution 15.00 ml - -
NaCl 3.46 g 3.379 57.818
Na2-EDTA 0.77 g 0.752 2.237
MgSO4 x 7 H2O 0.88 g 0.859 3.487
MgCl2 x 6 H2O 0.69 g 0.674 3.314
CaCl2 x 2 H2O 0.14 g 0.137 0.93
KCl 0.08 g 0.078 1.048
NaBr 12.50 mg 0.012 0.119
H3BO3
(0.1% w/v) 		4.00 ml 0.004 0.063
(NH4)2Ni(SO4)2
(0.1% w/v) 		3.00 ml 0.003 0.007
SrCl2 x 6 H2O
(0.1% w/v) 		1.90 ml 0.002 0.007
KI
(0.01% w/v) 		0.10 ml 9.8e-6 3.8e-5
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
KH2PO4 0.50 g 0.488 3.588
Starch
(soluble) 		5.00 g 4.883 -
Na2S x 9 H2O 0.50 g 0.488 2.033
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, starch and sulfide) and adjust pH to 7.0. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% volume and autoclave. Add hydrogenphosphate, starch and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of the complete medium and adjust to 7.0 - 7.2, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1
Pseudothermotoga thermarum DSM 5069

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Starch 4.883 -
NaCl 3.379 57.818
MgSO4 x 7 H2O 0.859 3.487
Na2-EDTA 0.752 2.237
MgCl2 x 6 H2O 0.674 3.314
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Cultivation metadata from 2 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain