DSMZ 502 . PSEUDOCLOSTRIDIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.20 g 1.2 20.534
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KCl 0.30 g 0.3 4.024
CaCl2 x 2 H2O 0.15 g 0.15 1.02
NH4Cl 0.27 g 0.27 5.048
KH2PO4 0.21 g 0.21 1.543
Na2SO4 0.10 g 0.1 0.704
Trace element solution SL-10 1.00 ml - -
Inulin 5.00 g 5 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Yeast extract 0.03 g 0.03 -
Casamino acids
(BD Bacto)
0.03 g 0.03 -
Wolin's vitamin solution 10.00 ml - -
Na2CO3 1.50 g 1.5 14.153
Na2HPO4 2.66 g 2.66 18.738
Na2S x 9 H2O 0.15 g 0.15 0.625
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except yeast extract, Casamino acids, vitamins, carbonate, hydrogenphosphate and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add yeast extract, Casamino acids, hydrogenphosphate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas atmosphere and strerilized by filtration. Adjust pH of complete medium to 7.0, if necessay.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Inulin 5 -
Na2HPO4 2.66 18.738
Na2CO3 1.5 14.153
NaCl 1.2 20.534
MgCl2 x 6 H2O 0.4 1.968
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains