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502. PSEUDOCLOSTRIDIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.20 g 1.186 20.29
2 0.40 g 0.395 1.944
3 0.30 g 0.296 3.976
4 0.15 g 0.148 1.008
5 0.27 g 0.267 4.988
6 0.21 g 0.208 1.525
7 0.10 g 0.099 0.696
8 Trace element solution SL-10 1.00 ml - -
9 5.00 g 4.941 -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 0.03 g 0.03 -
12
(BD Bacto)
0.03 g 0.03 -
13 Vitamin solution 10.00 ml - -
14 1.50 g 1.482 13.985
15 2.66 g 2.628 18.516
16 0.15 g 0.148 0.617
17 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except yeast extract, Casamino acids, vitamins, carbonate, hydrogenphosphate and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add yeast extract, Casamino acids, hydrogenphosphate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas atmosphere and strerilized by filtration. Adjust pH of complete medium to 7.0, if necessay.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
Inulin 4.941 -
Na2HPO4 2.628 18.516
Na2CO3 1.482 13.985
NaCl 1.186 20.29
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains

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